Journal: Addiction neuroscience

Article Title: Adolescent alcohol exposure reduces dopamine 1 receptor modulation of prelimbic neurons projecting to the nucleus accumbens and basolateral amygdala

doi: 10.1016/j.addicn.2022.100044

Figure Lengend Snippet: Dendritic spine morphometric analyses in PrL neurons that project to the NAc (PrL NAc ). (A) Surgery schematic depicting localization of retrograde Cre virus and Cre-dependent mCherry viral vector infusion sites. A pyramidal neuron is also depicted with boxes around the areas of the apical dendrite where spine segments were imaged (B,C) Representative images of proximal apical (B) and apical tuft dendritic segments (C). Top panel shows viral labeling in red with the dendrite of interest highlighted in yellow. The center panel shows the isolated dendrite pseudo colored in green. The bottom panel provides the digital spine analyses images with the shaft region shown in green and the spine region shown in blue. Dendritic spine density and head diameter data for the proximal apical segments (D) and the apical tuft segments (E). No significant differences were observed in spine density or head diameter at either the proximal or tuft regions of the apical dendrite when comparing Air (yellow circles) and AIE (blue squares) groups (unpaired t-tests). Data represent the mean ± SEM. n = 5-6 rats/group.

Article Snippet: Rats then received bilateral microinjection (0.75 μl/hemisphere) of Cre-dependent mCherry labeling vector (AAV2–hSyn–DIO–mCherry) obtained from Addgene into the prelimbic cortex (from bregma: +2.8 mm AP, ±0.6 mm ML, −3.8 mm DV).

Techniques: Virus, Plasmid Preparation, Labeling, Isolation